原著論文（査読制）*印は
Corresponding Author
(57)
Kitazaki C, Numano S, Takanezawa A, Nishizawa T, Shirai M,
*Asayama
M.
Characterization of Lysis of Multicellular
Cyanobacterium, Limnothrix/Pseudanabaena
sp.
Strain ABRG5-3.
Biosci.
Biotechnol. Biochem.,
(in
press)
(56)
Tazuke A, Asayama
M.
Expression of CsSEF1
gene
encoding putative CCCH zinc finger protein is induced by
defoliation and prolonged darkness in cucumber fruit.
Planta,
237:681-691 (2013)
(55)
*Asayama
M.
Overproduction and Easy Recovery of Target Gene Products
from Cyanobacteria, Photosynthesizing Microorganisms.
Appl. Microbiol. Biotechnol., 95:
683-695, DOI 10.1007/s00253-012-3989-0 (2012)
(54)
Nishizawa T, Ueda A, Nakano T, Nishizawa A, Miura T,
Asayama
M, Fujii
K, Harada K, Shirai M.
Characterization of the locus of genes encoding enzymes
producing heptadepsipeptide micropeptin in the unicellular
cyanobacterium Microcystis.
J. Biochem., 149:
475-85 (2011)
(53) Miura T, Hosoya Y, Yan-Zhuo Y, Nishizawa T,
Asayama
M,
Takahashi H, Shirai M.
In
vivo and
in
vitro characterization
of site-specific recombination of actinophage R4 integrase.
J. Gen. Appl. Microbiol., 57:
45-57 (2011)
(52) Nishizawa T, Hanami T, Hirano E, Miura T, Watanabe Y,
Tanakezawa A, Komatsuzaki M, Ohta H, Shirai M,
*Asayama
M.
Isolation and Molecular Characterization of a Multicellular
Cyanobacterium, Limnothrix/
Pseudanabaena
sp.
Strain ABRG5-3.
Biosci. Biotechnol. biochem.,
74:1827-1835 (2010)
(51) Imamura S, *Asayama
M.
Sigma Factors for Cyanobacterial Transcription.
Gene
Regulation and Systems Biology,
3:65-87 (2009)
(50) Nishizawa T, *Asayama
M, Shirai
M.
Natural product biosynthetic gene cluster from
cyanobacteria.
In
"Photosynthesis:
Theory and Applications in Energy, Biotechnology and
Nanotechnology" ed. by
Buchner TB and Ewingen NH.
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Science Publishers, Inc. (New York). pp.
227-237 (2009)
(49) Noguchi T, Shinohara A, Nishizawa A,
Asayama
M, Nakano
T, Hasegawa M, Harada K, Shirai M.
Genetic analysis of the microcystis biosynthesis gene
cluster in Microcystis
strains
from four bodies of
eutrophic water in Japan.
J.
Gen. Appl. Microbiol., 55:
111-123 (2009)
(48)
Osanai
T, Imashimizu M, Seki A, Sato S, Tabata S, Imamura
S, Asayama
M,
Ikeuchi M, Tanaka K.
ChlH,
the H subunit of the Mg-chelatase, is an anti-sigma factor
for SigE in Synechocystis sp. PCC
6803.
Proc.
Natl. Acad. Sci. U S A, 106:
6860-6865 (2009)
(47)
*Asayama
M,
Imamura S.
Stringent promoter recognition and autoregulation by the
group 3 sigma factor SigF
in the cyanobacterium Synechocystis
sp. PCC
6803.
Nucleic
Acids Res., 36:
5297-5305 (2008)
(46)
Saji S, Bathula S, Kubo A, Tamaoki M, Kanna M, Aono M,
Nakajima N, Nakaji T, Takeda T, Asayama
M,
Saji H.
Disruption of a gene encoding C4-dicarboxylate
transporter-like protein increases ozone sensitivity
through deregulation of the stomatal response in
Arabidopsis
thaliana.
Plant
Cell Physiol., 49:
2-10 (2008)
(45)
Nishizawa T, Nishizawa
A, Asayama
M, Harada
K, Shirai M.
Diversity within the microcystin biosynthetic gene clusters
among the genus Microcystis.
Microbes
and Environments, 22:
380-390 (2007)
(44)
Horie Y, Ito Y,
Ono M, Moriwaki N, Kato H, Hamakubo Y, Amano T, Wachi M,
Shirai M, *Asayama
M.
Dark-induced mRNA instability involves RNase E/G-type
endoribonuclease
cleavage at the AU-box and SD sequences in cyanobacteria.
Mol.
Genomics Genetics, 278:
331-346 (2007)
(43)
Saio T, Kumeta H, Ogura K, Yokochi M, Asayama
M, Katoh
S, Katoh E, Teshima K, Inagaki F.
The cooperative role of OsCnfU-1A Domain I and Domain II in
the iron sulphur cluster transfer process as revealed by
NMR.
J.
Biochem., 142:
113-121 (2007)
(42) Kumeta H, Ogura K, Asayama
M, Katoh
S, Katoh E, Teshima K, Inagaki F.
THe NMR structure of the domain II of a chloroplastic
NifU-like protein OsNifUA.
J. Biomol. NMR,
38:161-164 (2007)
(41)
Yoshimura T, Imamura S, Tanaka K, Shirai M,
*Asayama
M.
Cooperation of group 2 sigma factors, SigD and SigE for
light-induced transcription in the cyanobacterium
Synechocystis
sp. PCC
6803.
FEBS Lett., 581:
1495-1500 (2007)
(40)
Nishizawa A, Arshad
AB, Nishizawa T, Asayama
M, Fujii
K, Nakano T, Harada
K, Shirai M.
Cloning and Characterization of a new hetero-gene cluster
of nonribomal peptide synthetase and polyketide synthase
from the cyanobacterium Microcystis
aeruginosa K-139.
J.
Gen. Appl. Microbiol., 53:
17-27 (2007)
(39) Matsui M, Yoshimura T, Wakabayashi Y, Imamura S,
Tanaka K, Tanahashi H, Asayama
M, Shirai
M.
Interference
expression at levels of the transcript and protein among
group 1, 2, and 3 sigma
factor genes in a cyanobacterium.
Microbes and Environments, 22:
32-43 (2007)
(38)
Kubota Y, Miyao A, Hirochika H, Tozawa Y, Yasuda H,
Tsunoyama Y, Niwa Y, Imamura S, Shirai M,
*Asayama
M.
Two novel nuclear genes,
OsSIG5 and
OsSIG6,
encoding potential plastid sigma factors of RNA polymerase
in rice: tissue-specific and light-responsive
transcription.
Plant
Cell Physiol.,
48:186-192 (2007)
(37)
Osanai
T, Imamura S, Asayama
M, Shirai
M, Suzuki I, Murata N, Tanaka K.
Nitrogen induction of sugar catabolic gene expression
in Synechocystis
sp. PCC
6803.
DNA Res.,
13:185-195 (2006)
(36)
*Asayama
M.
Regulatory system for light-responsive gene expression in
photosynthesizing bacteria:
cis-elements
and trans-acting
factors in transcription and post-transcription.
Biosci.
Biotechnol. Biochem.,
70:565-573 (2006)
(35)
Imamura S, Tanaka K, Shirai M, *Asayama
M.
Growth
phase-dependent activation of nitrogen-related genes by a
control network of group 1 and group 2 sigma factors in a
cyanobacterium.
J. Biol. Chem.,
281:2668-2675 (2006)
(34)
Osanai T, Kanesaki Y, Nakano T, Takahashi H,
Asayama
M, Shirai
M, Kanehisa M, Suzuki I, Murata N, Tanaka
K.
Positive
regulation of sugar catabolic pathways in the
cyanobacterium Synechocystis
sp. PCC
6803 by the Group 2 factor SigE.
J.
Biol. Chem.,
280:30653-30659 (2005)
(33)
Machida M,
Nakajima S, Shikano N, Nishio J, Okada S,
Asayama
M, Shirai
M, Kubota N.
Heat shock protein 90 inhibitor
17-allylamino-17-demethoxygeldanamycin potentiates the
radiation response of tumor cells grown as monolayer
cultures and spheroids by inducing
apoptosis.
Cancer Sci., 96:
911-917 (2005).
(32)
Katoh S, Murata K, Kubota Y, Kumeta H, Ogura K, Inagaki
F, Asayama
M, Katoh,
E.
Refolding and purification of recombinant OsNifU1A domain
II that was expressed by Escherichia
coli.
Prot.
Exp. Purif.,
43:149-156 (2005)
(31)
Imamura S, *Asayama
M, Shirai
M.
In
vitro transcription
analysis by reconstituted cyanobacterial RNA polymerase:
roles of group 1and 2 sigma factors and a core subunit,
RpoC2.
Genes
Cells,
9:1175-1187 (2004)
(30)
*Asayama
M,
Imamura S, Yoshihara S, Miyazaki A, Yoshida N, Sazuka T,
Kaneko T, Ohara O,
Tabata S, Tanaka K, Takahashi H, Shirai M.
SigC, the group 2 sigma factor of RNA polymerase,
contributes to the late-stage expression and nitrogen
promoter recognition in the cyanobacterium
Synechocystis
sp.
strain PCC 6803.
Biosci.
Biotechnol. Biochem.,
68:477-487 (2004)
(29) Imamura S, *Asayama
M,
Hiroyuki Takahashi, Tanaka K, Takahashi H, Shirai M.
Antagonistic dark-/light-induced SigB/SigD expression
through redox potential and their roles as group 2 sigma
factors of RNA polymerase in Synechocystis
sp. PCC
6803.
FEBS
Lett., 554:357-362
(2003)
(28)
Agrawal GK, Asayama
M, Shirai
M.
Two-distinct curved DNAs upstream of the
light-responsive psbA
gene in
a cyanobacterium.
Biosci.
Biotechnol. Biochem.,
67:1817-1821 (2003)
(27) Ito
Y, *Asayama
M, Shirai
M.
Light-responsive psbA
transription
requires the –35 hexamer in the promoter and its proximal
upstream element, UPE, in cyanobacteria.
Biosci.
Biotechnol. Biochem.,
67:1382-1390 (2003)
(26) Imamura
S, Yoshihara S, Nakano S, Shiozaki N, Yamada A, Tanaka K,
Takahashi H, Asayama
M, Shirai
M.
Purification, characterization, and gene expression of all
sigma factors of RNA polymerase in a cyanobacterium.
J.
Mol. Biol.,
325:857-872 (2003)
(25)
Asayama
M, Kato
H, Shibato J, Shirai M, Ohyama T.
The curved DNA structure in the 5’-upstream region of the
light-responsive genes: its universality, binding factor
and function for cyanobacterial psbA
transcription.
Nucleic Acids Res., 30:
4658-4666 (2002)
(24)
Shibato J, Agrawal GK, Kato H, Asayama
M, Shirai
M.
The 5’-upstream cis-sequences
of cyanobacterial psbA:
analysis of their roles in basal, light-dependent and
circadian rhythmic transcription.
Mol. Genet. Genomic.,
267:684-694 (2002)
(23)
Agrawal GK, Kato H, Asayama
M, Shirai
M.
An AU-box motif upstream of the SD-seqence of
light-dependent psbA
transcripts
confers mRNA instability under darkness in
cyanobacteria.
Nucleic Acids Res., 29:
1835-1843 (2001)
(22)
Nishizawa
T, Asayama
M, Shirai
M.
Cyclic heptapeptide microcystin biosynthesis requires the
glutamate racemase.
Microbiol. 147:1235-1241
(2001)
(21)
Nishizawa
T, Ueda A, Asayama
M, Fujii
K, Harada K, Ochi K, Shirai M.
Polyketide synthase gene coupled to the peptide synthetase
module involved in the biosynthesis of the cyclic
heptapeptide Microcystin.
J. Biochem.,
127:779-789 (2000)
(20)
Asayama
M,
Hayasaka Y, Kabasawa M, Shirai M, Ohyama T.
An intrinsic DNA curvature found in the
cyanobacterium Microcystis
aeruginosa K-81
affects the promoter activity of rpoD1
encoding
a principal sigma factor.
J. Biochem.,
125:460-468 (1999)
(19)
Agrawal
GK, Asayama
M, Shirai
M.
Light-dependent
and rhythmic psbA
transcripts
in homologous/heterologous cyanabacterial cells.
Biochem. Biophys. Res. Commun., 255:
47-53 (1999)
(18)
Nishizawa T, Asayama
M, Fujii
K, Harada K, Shirai M.
Genetic analysis of the peptide synthetase genes for a
cyclic heptapeptide Microcystin
in
Microsystis
spp.
J. Biochem.,
126:520-529 (1999)
(17)
Asayama
M, Saito
K, Kobayashi Y.
Translational attenuation of the Bacillus
subtilis spo0B
cistron
by an RNA structure encompassing the initiation region.
Nucleic Acids Res.,
26:824-830 (1998)
(16)
Shibato
J, Asayama
M, Shirai
M.
Specific recognition of the cyanobacterial
psbA
promoter
by RNA polymerases containing principal sigma
factors.
Biochim. Biophys Acta, 1442:
296-303 (1998)
(15)
Asayama
M, Suzuki
A, Nozawa S, Yamada A, Tanaka K, Takahashi H, Aida T,
Shirai M.
A new sigma factor homolog in a cyanobacterium: cloning,
sequencing, and light-responsive transcripts of
rpoD2
from
Microcystis
aeruginosa K-81.
Biochim. Biophys. Acta,
1351:31-36 (1997)
(14)
Asayama
M,
Kabasawa M, Shirai M.
A novel
genetic organization: the leuA-rpoD1
locus in
the cyanobacterium Microcystis
aeruginosa K-81.
Biochim. Biophys. Acta, 1350:15-20
(1997)
(13)
Agrawal GK, Asayama
M, Shirai
M.
A novel
bend of DNA CIT:
changeable
bending-center sites of an intrinsic
curvature under temperature
conditions.
FEMS Microbiol. Lett.,
147:139-145 (1997)
(12)
Asayama
M, Yamada
A, Tanaka K, Takahashi H, Shirai, M.
A new
set of PCR primers for specific detection of the gene
encoding the principal sigma factor in
cyanobacteria.
J. Gen. Appl. Microbiol.,
42:511-515 (1996)
(11)
Sato M, Shibato J, Aida T, Asayama
M, Shirai
M.
Light-responsive
and rhythmic gene expression of psbA2
in
cyanobacterium Microcystis
aeruginosa K-81.
J. Gen. Appl. Microbiol.,
42:381-391 (1996)
(10)
Asayama
M, Suzuki
H, Sato A, Aida T, Tanaka K, Takahashi H, Shirai M.
The
rpoD1
gene
product is a principal sigma factor of RNA polymerase
in Microcystis
aeruginosa
K-81.
J. Biochem.,
120:752-758 (1996)
(9)
Asayama
M, Tanaka
K, Takahashi H, Sato A, Aida T, Shirai M.
Cloning,
sequencing and characterization of the gene encoding a
principal sigma factor homolog from the
cyanobacterium Microcystis
aeruginosa K-81.
Gene,
181:213-217 (1996)
(8)
Asayama
M,
Kabasawa M, Takahashi I, Aida T, Shirai, M.
Highly
repetitive sequences and characteristics of genomic DNA in
unicellular cyanobacterial strains.
FEMS Mirobiol. Lett.,
137:175-181 (1996)
(7)
Takahashi I, Hayano D, Asayama
M,
Masahiro F, Watahiki M, Shirai M.
Restriction
barrier composed of an extracellular nuclease and
restriction endonuclease in the unicellular
cyanobacterium Microcystis
sp.
FEMS Microbiol. Lett.,
145:107-111 (1996)
(6)
Matsuura M, Noguchi T, Yamaguchi D, Aida T,
Asayama
M,
Takahashi H, Shirai M. The sre
gene
(ORF469) encodes a site-specific recombinase responsible
for integration of the R4 phage genome.
J. Bacteriol.,
178:3374-3376 (1996)
(5)
Asayama
M,
Yamamoto A, Kobayashi Y.
Dimer
form of phosphorylated Spo0A, a transcriptional regulator,
stimulates the spo0F
transcription
at the initiation of sporulation in Bacillus
subtilis.
J. Mol. Biol.,
250:11-23 (1995)
(4)
Matsuura
M, Noguchi T, Aida T, Asayama
M,
Takahashi H, Shirai M. A gene essential for the
site-specific excision of actinophage R4 prophage genome
from the chromosome of a lysogen.
J. Gen. Appl. Microbiol.,
41:53-61 (1995)
(3)
Sakamoto T, Shirai M, Asayama
M, Aida
T, Sato A, Tanaka K, Takahashi H, Nakano M.
Characteristics of DNA and multiple rpoD
homologs
of Microcystis(Synechocystis)
strains.
Int. J. Syst. Bacteriol.,
43:844-847 (1993)
(2)
Asayama
M., and
Kobayashi Y.
Signal
transduction and sporulation in Bacillus
subtilis :
Heterologous phosphorylation of Spo0A, a sporulation
initiation gene product.
J. Biochem.,
114:385-388 (1993)
(1)
Asayama
M,
Kobayashi Y.
Signal
transduction and sporulation in Bacillus
subtilis :
Autophosphorylation of Spo0A, a sporulation initiation gene
product.
Mol. Gen. Genet.,
238:138-144 (1993)